biotinylated secondary igg Search Results


biotinylated secondary ab (anti-mouse igg  (Becton Dickinson)
90
Becton Dickinson biotinylated secondary ab (anti-mouse igg
The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
Biotinylated Secondary Ab (Anti Mouse Igg, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated anti-igg secondary antibody  (Becton Dickinson)
90
Becton Dickinson biotinylated anti-igg secondary antibody
The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
Biotinylated Anti Igg Secondary Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated secondary antibody zsgb-bio, beijing, china  (ZSGB Biotech)
90
ZSGB Biotech biotinylated secondary antibody zsgb-bio, beijing, china
The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
Biotinylated Secondary Antibody Zsgb Bio, Beijing, China, supplied by ZSGB Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti-rabbit biotinylated secondary antibody biostain rabbit igg [ap] kit  (Biomeda corporation)
90
Biomeda corporation goat anti-rabbit biotinylated secondary antibody biostain rabbit igg [ap] kit
The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
Goat Anti Rabbit Biotinylated Secondary Antibody Biostain Rabbit Igg [Ap] Kit, supplied by Biomeda corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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secondary biotinylated igg antibodies  (EnoGene Inc)
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EnoGene Inc secondary biotinylated igg antibodies
The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
Secondary Biotinylated Igg Antibodies, supplied by EnoGene Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated goat anti-rabbit igg secondary antibody  (Oncogene Science Inc)
90
Oncogene Science Inc biotinylated goat anti-rabbit igg secondary antibody
The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
Biotinylated Goat Anti Rabbit Igg Secondary Antibody, supplied by Oncogene Science Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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secondary antibody (goat antirabbit biotinylated anti-igg  (Cosmo Bio USA)
90
Cosmo Bio USA secondary antibody (goat antirabbit biotinylated anti-igg
The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
Secondary Antibody (Goat Antirabbit Biotinylated Anti Igg, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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secondary biotinylated igg  (ScyTek Inc)
90
ScyTek Inc secondary biotinylated igg
The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
Secondary Biotinylated Igg, supplied by ScyTek Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+secondary+igg/secondary+biotinylated+igg/pm17785353-112-7-9
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secondary biotinylated goat anti-mouse igg  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology secondary biotinylated goat anti-mouse igg
The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
Secondary Biotinylated Goat Anti Mouse Igg, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+secondary+igg/secondary+biotinylated+goat+anti+mouse+igg/10__21608_slash_ejh__2020__22098__1229-74-11-15
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anti-rabbit igg biotin  (Linaris GmbH)
90
Linaris GmbH anti-rabbit igg biotin
The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
Anti Rabbit Igg Biotin, supplied by Linaris GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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secondary antibody biotinylated sheep anti-rabbit igg  (Beijing CWBio)
90
Beijing CWBio secondary antibody biotinylated sheep anti-rabbit igg
The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
Secondary Antibody Biotinylated Sheep Anti Rabbit Igg, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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secondary biotinylated horse anti-mouse igg solution #zb-2020  (ZSGB Biotech)
90
ZSGB Biotech secondary biotinylated horse anti-mouse igg solution #zb-2020
The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
Secondary Biotinylated Horse Anti Mouse Igg Solution #Zb 2020, supplied by ZSGB Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with biotinylated cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.

Journal:

Article Title: Inhibition of endogenous thioredoxin in the heart increases oxidative stress and cardiac hypertrophy

doi: 10.1172/JCI200317700

Figure Lengend Snippet: The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. (a) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK (a), Raf-1 (b), p38-MAPK (e), and p46/p54–JNK1 (f) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. (e) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. (c) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. (d) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with biotinylated cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.

Article Snippet: After washing, biotinylated secondary Ab (anti-mouse IgG; BD Pharmingen) was applied for 1 hour, followed by streptavidin-HRP (BD Pharmingen) for 30 minutes at room temperature.

Techniques: Positive Control, Western Blot, Activity Assay, Binding Assay, Transfection, Expressing, Incubation, Isolation